Small Molecule Inhibitors of the Sars-Cov Nsp15
Small Molecule Inhibitors of the Sars-Cov Nsp15
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Author: Joanna Ortiz-Alcantara
Page Count: 68 pages
Published Date: 05 Mar 2012
Publisher: LAP Lambert Academic Publishing
Publication Country: Germany
Language: English
Format: Pdf
ISBN: 9783848407637
File Name: Small.Molecule.Inhibitors.of.the.Sars-Cov.Nsp15.pdf
Download Link: Small Molecule Inhibitors of the Sars-Cov Nsp15
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The Severe Acute Respiratory Syndrome (SARS) virus encodes several unusual RNA processing enzymes, including Nsp15, an endoribonuclease that cleaves 3' of uridylates through a Ribonuclease A-like mechanism. Crystal structures confirmed that the Nsp15 active site is structurally similar to that of Ribonuclease A. These similarities and our molecular docking analysis lead us to hypothesize that previously characterized Ribonuclease A inhibitors will also inhibit the SARS-CoV Nsp15. Benzopurpurin B, C-467929, C-473872, N-36711, N-65828, N-103018 and Congo red were tested for effects on Nsp15 endoribonuclease activity. A real-time fluorescence assay revealed IC50 values for inhibiting Nsp15 between 0.2 M and 40 M. Benzopurpurin B, C-473872, and Congo red are competitive inhibitors, according to kinetic studies and bind SARS-CoV Nsp15 according to a differential scanning fluorimetry assay. Benzopurpurin B also inhibited the Nsp15 orthologs from two other coronaviruses: mouse hepatitis virus (MHV) and infectious bronchitis virus. The three compounds reduced infectivity of MHV in L2 cells by 8 to 26 fold. The more effective drugs also drecreased RNA accumulation in MHV.
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Small Molecule Inhibitors of the Sars-Cov Nsp15 by Joanna Ortiz-Alcantara
Author: Joanna Ortiz-Alcantara
Page Count: 68 pages
Published Date: 05 Mar 2012
Publisher: LAP Lambert Academic Publishing
Publication Country: Germany
Language: English
Format: Pdf
ISBN: 9783848407637
File Name: Small.Molecule.Inhibitors.of.the.Sars-Cov.Nsp15.pdf
Download Link: Small Molecule Inhibitors of the Sars-Cov Nsp15
---------------------------------------------------------------
The Severe Acute Respiratory Syndrome (SARS) virus encodes several unusual RNA processing enzymes, including Nsp15, an endoribonuclease that cleaves 3' of uridylates through a Ribonuclease A-like mechanism. Crystal structures confirmed that the Nsp15 active site is structurally similar to that of Ribonuclease A. These similarities and our molecular docking analysis lead us to hypothesize that previously characterized Ribonuclease A inhibitors will also inhibit the SARS-CoV Nsp15. Benzopurpurin B, C-467929, C-473872, N-36711, N-65828, N-103018 and Congo red were tested for effects on Nsp15 endoribonuclease activity. A real-time fluorescence assay revealed IC50 values for inhibiting Nsp15 between 0.2 M and 40 M. Benzopurpurin B, C-473872, and Congo red are competitive inhibitors, according to kinetic studies and bind SARS-CoV Nsp15 according to a differential scanning fluorimetry assay. Benzopurpurin B also inhibited the Nsp15 orthologs from two other coronaviruses: mouse hepatitis virus (MHV) and infectious bronchitis virus. The three compounds reduced infectivity of MHV in L2 cells by 8 to 26 fold. The more effective drugs also drecreased RNA accumulation in MHV.
Read online Small Molecule Inhibitors of the Sars-Cov Nsp15 Buy Small Molecule Inhibitors of the Sars-Cov Nsp15 Download and read Small Molecule Inhibitors of the Sars-Cov Nsp15 ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent Download to iPad/iPhone/iOS, B&N nook Small Molecule Inhibitors of the Sars-Cov Nsp15 ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent
The Verdict of a Closed Case ebook
Download PDF, EPUB, MOBI Visigothic Kingdom : France, Iberian Peninsula, Germanic Peoples, Western Roman Empire, Wallia, Aquitaine
Download ebook Feminine Image in Literature
keyseater machines with adjustable y axis
Implementing Lean Manufacturing : An Operations Managers Guide download PDF, EPUB, Kindle